Epigenetics & Nuclear Signaling Antibodies 4

Anti-Exonuclease V Antibody (CAB8507)

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SKU:
CAB8507
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Exonuclease V Antibody
Antibody SKU:CAB8507
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human EXO5 (NP_073611.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:THP-1, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human EXO5 (NP_073611.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAET REEE TVSA EASG FSDL SDSE FLEF LDLE DAQE SKAL VNMP GPSS ESLG KDDK PISL QNWK RGLD ILSP MERF HLKY LYVT DLAT QNWC ELQT AYGK ELPG FLAP EKAA VLDT GASI HLAR ELEL HDLV TVPV TTKE DAWA IKFL NILL LIPT LQSE GHIR EFPV FGEG EGVL LVGV IDEL HYTA KGEL ELAE LKTR RRPM LPLE AQKK KDCF QVSL YKYI FDAM VQGK VTPA SLIH HTKL CLEK PLGP SVLR HAQQ GGFS VKSL GDLM ELVF LSLT LSDL PVID ILKI EYIH QETA TVLG TEIV AFKE KEVR AKVQ HYMA YWMG HREP QGVD VEEA WKCR TCTY ADIC EWRK GSGV LSST LAPQ VKKA K
Gene ID:64789
Uniprot:Q9H790
Cellular Location:Cytoplasm, Nucleus, cytosol
Calculated MW:41kDa
Observed MW:42kDa
Synonyms:EXO5, C1orf176, DEM1, Exo V, hExo5, ExoV
Background:
UniProt Protein Function:DEM1: Probable single strand DNA specific 5' exonuclease mitochondrial DNA replication and recombination. Belongs to the EXO5 family.
UniProt Protein Details:

Protein type:EC 3.1.11.-

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:4 iron, 4 sulfur cluster binding; DNA binding; metal ion binding; protein homodimerization activity; single-stranded DNA specific 3'-5'exodeoxyribonuclease activity; single-stranded DNA specific 5'-3'exodeoxyribonuclease activity

Biological Process: nucleic acid phosphodiester bond hydrolysis

NCBI Summary:The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage. [provided by RefSeq, Nov 2016]
UniProt Code:Q9H790
NCBI GenInfo Identifier:1101414072
NCBI Gene ID:64789
NCBI Accession:NP_001333875.1
UniProt Secondary Accession:Q9H790,Q5SWM7, Q5SWM8, Q5SWM9, Q5SWN0, Q5SWN1, Q8WTW9 D3DPV4,
UniProt Related Accession:Q9H790
Molecular Weight:41,816 Da
NCBI Full Name:exonuclease V
NCBI Synonym Full Names:exonuclease 5
NCBI Official Symbol:EXO5  
NCBI Official Synonym Symbols:DEM1; Exo V; hExo5; C1orf176  
NCBI Protein Information:exonuclease V
UniProt Protein Name:Exonuclease V
UniProt Synonym Protein Names:Defects in morphology protein 1 homolog
Protein Family:Probable exonuclease
UniProt Gene Name:EXO5  
Anti-Exonuclease V Polyclonal Antibody (CAB8507)Western blot analysis of extracts of various cell lines, using EXO5 antibody (CAB8507) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 1s.
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