Cardiovascular Antibodies

Annexin A5/Annexin V Rabbit Monoclonal Antibody (CAB9262)

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SKU:
CAB9262
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cardiovascular
Reactivity:
Human, Mouse, Rat
Synonyms:
PP4, ANX5, ENX2, CPB-I, RPRGL3, HEL-S-7, VAC-alph, Annexin A5/Annexin V
Research Use:
Cardiovascular
Frequently bought together:

Description

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Annexin A5/Annexin V Monoclonal Antibody (CAB9262)

The Annexin A5/Annexin V Monoclonal Antibody (CAB9262) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been extensively validated for use in Western blot and immunohistochemistry applications.Annexin V is a key player in the process of programmed cell death, serving as a marker for apoptotic cells. By targeting Annexin V with this antibody, researchers can accurately detect and analyze apoptotic cells in various experimental settings, providing valuable insights into cell death mechanisms and signaling pathways.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Annexin A5/Annexin V Monoclonal Antibody
SKU:CAB9262
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1499
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:IEET IDRE TSGN LEQL LLAV VKSI RSIP AYLA ETLY YAMK GAGT DDHT LIRV MVSR SEID LFNI RKEF RKNF ATSL YSMI KGDT SGDY KKAL LLLC GEDD
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PP4, ANX5, ENX2, CPB-I, RPRGL3, HEL-S-7, VAC-alph, Annexin A5/Annexin V
Positive Sample:HeLa, Hep G2, C6, Mouse brain, Rat lung
Cellular Localization:Cytoplasm, Cytosol, External Side Of Plasma Membrane, Extracellular Exosome, Extracellular Region, Focal Adhesion.
Calculated MW:36kDa
Observed MW:36kDa

The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications.

Purification MethodAffinity purification
Gene ID308
RRIDAB_2863698
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Annexin A5/Annexin A5/Annexin V Rabbit mAb (CAB9262) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using Annexin A5/Annexin A5/Annexin V Rabbit mAb (CAB9262) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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