Cell Biology Antibodies 12

EPS15 Rabbit Polyclonal Antibody (CAB9814)

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SKU:
CAB9814
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
AF1P, AF-1P, MLLT5, EPS15
Research Use:
Cell Biology
Frequently bought together:

Description

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EPS15 Antibody (CAB9814)

The EPS15 Antibody (CAB9814) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards EPS15 in human samples, making it ideal for Western blot applications.EPS15 is involved in regulating the internalization of cell surface receptors and the formation of clathrin-coated pits, essential processes for cell signaling and homeostasis. Dysregulation of EPS15 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular function.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:EPS15 Antibody
SKU:CAB9814
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RQST DPFA TSST DPFS AANN SSIT SVET LKHN DPFA PGGT VVAA SDSA TDPF ASVF GNES FGGG FADF STLS KVNN EDPF RSAT SSSV SNVV ITKN VFEE TSVK SEDE PPAL PPKI GTPT RPCP LPPG KRSI NKLD SPDP FKLN DPFQ PFPG NDSP KEKD PEIF CDPF TSAT TTTN KEAD P
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AF1P, AF-1P, MLLT5, EPS15
Positive Sample:HeLa, Jurkat, 293T, NIH/3T3, Mouse liver, Mouse spleen, Mouse lung
Cellular Localization:Cell Membrane, Cytoplasm, Cytoplasmic Side, Early Endosome Membrane, Membrane, Peripheral Membrane Protein, Clathrin-Coated Pit.
Calculated MW:99kDa
Observed MW:150kDa

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification MethodAffinity purification
Gene ID2060
RRIDAB_2769344
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using EPS15 Rabbit pAb (CAB9814) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Product ImageImmunofluorescence analysis of C6 cells using EPS15 Rabbit pAb (CAB9814) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using EPS15 Rabbit pAb (CAB9814) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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