Cell Biology Antibodies 6

eNOS Rabbit Polyclonal Antibody (CAB1548)

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SKU:
CAB1548
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
eNOS, ECNOS
Research Use:
Cell Biology
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Description

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eNOS Antibody (CAB1548)

The eNOS Antibody (CAB1548) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through rabbit immunization, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to NOS3, this antibody enables the detection and analysis of the enzyme in various cell types, facilitating research in cardiovascular physiology and pathology.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:eNOS Antibody
SKU:CAB1548
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RLPP DPSL PCIL VGPG TGIA PFRG FWQE RLHD IESK GLQP TPMT LVFG CRCS QLDH LYRD EVQN AQQR GVFG RVLT AFSR EPDN PKTY VQDI LRTE LAAE VHRV LCLE RGHM FVCG DVTM ATNV LQTV QRIL ATEG DMEL DEAG DVIG VLRD QQRY HEDI FGLT LRTQ EVTS RIRT QSFS LQER QLRG AVPW AFDP PGSD TNSP
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:eNOS, ECNOS
Positive Sample:Rat placenta, Rat heart
Cellular Localization:Cell Membrane, Cytoplasm, Golgi Apparatus, Membrane, Caveola, Cytoskeleton.
Calculated MW:133kDa
Observed MW:140kDa

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID4846
RRIDAB_2762878
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using eNOS Rabbit pAb (CAB1548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of paraffin-embedded human placenta using eNOS Rabbit pAb (CAB1548) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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