Cell Biology Antibodies 6

CGN Rabbit Polyclonal Antibody (CAB15489)

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SKU:
CAB15489
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Synonyms:
CGN, cingulin
Research Use:
Cell Biology
Frequently bought together:

Description

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CGN Antibody (CAB15489)

The CGN Antibody (CAB15489) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been rigorously validated for Western blot applications. By specifically targeting the CGN protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology, cell signaling, and cancer research.CGN, also known as cingulin, plays a crucial role in cell adhesion and signal transduction pathways, making it a key player in maintaining cellular function and integrity.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:CGN Antibody
SKU:CAB15489
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:TSGG LSRL QDEI QRLR QALQ ASQA ERDT ARLD KELL AQRL QGLE QEAE NKKR SQDD RARQ LKGL EEKV SRLE TELD EEKN TVEL LTDR VNRG RDQV DQLR TELM QERS ARQD LECD KISL ERQN KDLK TRLA SSEG FQKP SASL SQLE SQNQ LLQE RLQA EERE KTVL QSTN RKLE RKVK ELSI QIED ERQH VNDQ KDQL SLRV KALK RQVD EAEE EIER LDGL RKKA QREV EEQH EVNE QLQA RIKS LEKD SWRK ASRS AAES ALKN EGLS SDEE FDSV YDPS SIAS LLTE SNLQ TSSC
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:200 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CGN, cingulin
Positive Sample:BxPC-3, 293T, LO2, A-549
Cellular Localization:Cell Junction, Tight Junction.
Calculated MW:137kDa
Observed MW:160kDa

Enables cadherin binding activity. Predicted to act upstream of or within bicellular tight junction assembly; epithelial cell morphogenesis; and microtubule cytoskeleton organization. Located in bicellular tight junction and plasma membrane.

Purification MethodAffinity purification
Gene ID57530
RRIDAB_2762888
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CGN Rabbit pAb (CAB15489) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CGN Rabbit pAb (CAB15489) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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