Immunology Antibodies 2

eIF4A1 Rabbit Polyclonal Antibody (CAB5294)

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SKU:
CAB5294
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
DDX2A, EIF4A, EIF-4A, eIF4A-I, eIF-4A-I, eIF4A1
Research Use:
Immunology
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Description

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eIF4A1 Antibody (CAB5294)

The eIF4A1 Antibody (CAB5294) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the EIF4A1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.EIF4A1 is a critical component of the eukaryotic translation initiation complex, playing a pivotal role in mRNA unwinding and ribosome scanning during protein synthesis.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:eIF4A1 Antibody
SKU:CAB5294
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSAS QDSR SRDN GPDG MEPE GVIE SNWN EIVD SFDD MNLS ESLL RGIY AYGF EKPS AIQQ RAIL PCIK GYDV IAQA QSGT GKTA TFAI SILQ QIEL DLKA
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DDX2A, EIF4A, EIF-4A, eIF4A-I, eIF-4A-I, eIF4A1
Positive Sample:HepG2, 293T, C2C12, C6, Mouse brain
Cellular Localization:Cytoplasm, Cytosol, Eukaryotic Translation Initiation Factor 4F Complex, Extracellular Exosome.
Calculated MW:46kDa
Observed MW:48kDa

Enables double-stranded RNA binding activity. Predicted to be involved in cytoplasmic translational initiation. Located in cytoplasm.

Purification MethodAffinity purification
Gene ID1973
RRIDAB_2766106
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using eIF4A1 Rabbit pAb (CAB5294) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb (CAB5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb (CAB5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb (CAB5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb (CAB5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg eIF4A1 antibody (CAB5294). Western blot was performed from the immunoprecipitate using eIF4A1 antibody (CAB5294) at a dilution of 1:1000.
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