Metabolism Antibodies 2

EIF2B2 Rabbit Polyclonal Antibody (CAB7027)

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SKU:
CAB7027
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
EIF2B, EIF2Bbeta, EIF-2Bbeta, EIF2B2
Research Use:
Metabolism
Frequently bought together:

Description

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EIF2B2 Antibody (CAB7027)

The EIF2B2 Antibody (CAB7027) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the EIF2B2 protein, researchers can accurately detect and analyze this important molecule in a variety of cell types.EIF2B2, a subunit of the eukaryotic translation initiation factor 2B, plays a crucial role in the control of protein synthesis under conditions of cellular stress.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:EIF2B2 Antibody
SKU:CAB7027
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPGS AAKG SELS ERIE SFVE TLKR GGGP RSSE EMAR ETLG LLRQ IITD HRWS NAGE LMEL IRRE GRRM TAAQ PSET TVGN MVRR VLKI IREE YGRL HGRS DESD QQES LHKL LTSG GLNE
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EIF2B, EIF2Bbeta, EIF-2Bbeta, EIF2B2
Positive Sample:Mouse thymus, Rat kidney
Cellular Localization:Cytoplasm, Cytosol, Eukaryotic Translation Initiation Factor 2B Complex.
Calculated MW:39kDa
Observed MW:39kDa

This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation.

Purification MethodAffinity purification
Gene ID8892
RRIDAB_2767582
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using EIF2B2 Rabbit pAb (CAB7027) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using EIF2B2 Rabbit pAb (CAB7027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using EIF2B2 Rabbit pAb (CAB7027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using EIF2B2 Rabbit pAb (CAB7027) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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