Metabolism Antibodies 2

EEF1D Rabbit Polyclonal Antibody (CAB2509)

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SKU:
CAB2509
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
EF1D, EF-1D, FP1047, EEF1D
Research Use:
Metabolism
Frequently bought together:

Description

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EEF1D Antibody (CAB2509)

The EEF1D Antibody (CAB2509) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been extensively validated for use in Western blot applications, showing high reactivity with human samples. By specifically binding to EEF1D, this antibody allows for the accurate detection and analysis of the protein in various cell types.EEF1D, also known as elongation factor 1 delta, plays a crucial role in the elongation phase of protein synthesis and has been implicated in various cellular processes. Its role in translation regulation and signal transduction pathways make it a key player in cancer research, neurodegenerative diseases, and other disorders involving aberrant protein synthesis.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:EEF1D Antibody
SKU:CAB2509
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MATN FLAH EKIW FDKF KYDD AERR FYEQ MNGP VAGA SRQE NGAS VILR DIAR AREN IQKS LAGS SGPG ASSG TSGD HGEL VVRI ASLE VENQ SLRG VVQE LQQA ISKL EARL NVLE KSSP GHRA TAPQ TQHV SPMR QVEP PAKK PATP AEDD EDDD IDLF
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EF1D, EF-1D, FP1047, EEF1D
Positive Sample:MCF7, Mouse testis, jurkat, Jurkat
Cellular Localization:Nucleus.
Calculated MW:31kDa
Observed MW:40kDa/35kDa/40kDa

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Purification MethodAffinity purification
Gene ID1936
RRIDAB_2764400
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using EEF1D Rabbit pAb (CAB2509) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver tissue using EEF1D Rabbit pAb (CAB2509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EEF1D Rabbit pAb (CAB2509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EEF1D Rabbit pAb (CAB2509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of PC-12 cells using EEF1D Rabbit pAb (CAB2509) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit pAb (CAB2509). Western blot analysis was performed using EEF1D Rabbit pAb (CAB2509) at 1:500 dilution.
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