Name: Anti-EDARADD Antibody (CAB15950)
Brand: Developmental Biology
SKU: CAB15950
Availability: OutOfStock

Antibody Name:	Anti-EDARADD Antibody
Antibody SKU:	CAB15950
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human EDARADD (NP_665860.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	A-431, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human EDARADD (NP_665860.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGLR TTKQ MGRG TKAP GHQE DHMV KEPV EDTD PSTL SFNM SDKY PIQD TELP KAEE CDTI TLNC PRNS DMKN QGEE NGFP DSTG DPLP EISK DNSC KENC TCSS CLLR APTI SDLL NDQD LLDV IRIK LDPC HPTV KNWR NFAS KWGM SYDE LCFL EQRP QSPT LEFL LRNS QRTV GQLM ELCR LYHR ADVE KVLR RWVD EEWP KRER GDPS RHF
Gene ID:	128178
Uniprot:	Q8WWZ3
Cellular Location:	Cytoplasm
Calculated MW:	23kDa/24kDa
Observed MW:	25kDa

Synonyms:

EDARADD, ECTD11A, ECTD11B, ED3, EDA3

Background:

This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

UniProt Protein Function:	EDARADD: Adapter protein that interacts with EDAR DEATH domain and couples the receptor to EDA signaling pathway during morphogenesis of ectodermal organs. Mediates the activation of NF- kappa-B. Defects in EDARADD are a cause of ectodermal dysplasia anhidrotic (EDA); also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1q42.3 Cellular Component: cytosol Molecular Function:protein binding Biological Process: tumor necrosis factor-mediated signaling pathway Disease: Ectodermal Dysplasia 10a, Hypohidrotic/hair/nail Type, Autosomal Dominant; Ectodermal Dysplasia 10b, Hypohidrotic/hair/tooth Type, Autosomal Recessive; Ectodermal Dysplasia 11a, Hypohidrotic/hair/tooth Type, Autosomal Dominant; Ectodermal Dysplasia 11b, Hypohidrotic/hair/tooth Type, Autosomal Recessive
NCBI Summary:	This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WWZ3
NCBI GenInfo Identifier:	124504411
NCBI Gene ID:	128178
NCBI Accession:	BC128082
UniProt Secondary Accession:	Q8WWZ3,Q5VYJ7, A2VCK5, A8K7B5, B1AL54, B9ZVW5,
UniProt Related Accession:	Q8WWZ3
Molecular Weight:	23,690 Da
NCBI Full Name:	Homo sapiens EDAR-associated death domain, mRNA
NCBI Synonym Full Names:	EDAR associated death domain
NCBI Official Symbol:	EDARADD
NCBI Official Synonym Symbols:	ED3; EDA3; ECTD11A; ECTD11B
NCBI Protein Information:	ectodysplasin-A receptor-associated adapter protein
UniProt Protein Name:	Ectodysplasin-A receptor-associated adapter protein
UniProt Synonym Protein Names:	EDAR-associated death domain protein; Protein crinkled homolog
Protein Family:	Ectodysplasin-A receptor-associated adapter protein
UniProt Gene Name:	EDARADD
UniProt Entry Name:	EDAD_HUMAN

Western blot analysis of extracts of various cell lines, using EDARADD antibody (CAB15950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-EDARADD Antibody (CAB15951)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)