Cell Cycle Antibodies 1
Anti-E2F8 Antibody (CAB1135)
- SKU:
- CAB1135
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-E2F8 Antibody |
| Antibody SKU: | CAB1135 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human E2F8 (NP_078956.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human E2F8 (NP_078956.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MENE KENL FCEP HKRG LMKT PLKE STTA NIVL AEIQ PDFG PLTT PTKP KEGS QGEP WTPT ANLK MLIS AVSP EIRN RDQK RGLF DNRS GLPE AKDC IHEH LSGD EFEK SQPS RKEK SLGL LCHK FLAR YPNY PNPA VNND ICLD EVAE ELNV ERRR IYDI VNVL ESLH MVSR LAKN RYTW HGRH NLNK TLGT LKSI GEEN KYAE QIMM IKKK EYEQ EFDF IKSY SIED HIIK SNTG PNGH |
| Gene ID: | 79733 |
| Uniprot: | A0AVK6 |
| Cellular Location: | Nucleus |
| Calculated MW: | 94kDa |
| Observed MW: | 82kDa |
| Synonyms: | E2F8, E2F-8 |
| Background: | This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | E2F8: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Belongs to the E2F/DP family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 11p15.1 Cellular Component: transcription factor complex; nucleus Molecular Function:protein binding; protein homodimerization activity; transcription corepressor activity; transcription factor activity Biological Process: cell proliferation; transcription, DNA-dependent; positive regulation of DNA endoreduplication; positive regulation of transcription from RNA polymerase II promoter; sprouting angiogenesis; cell cycle comprising mitosis without cytokinesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cytokinesis; placenta development |
| NCBI Summary: | This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012] |
| UniProt Code: | A0AVK6 |
| NCBI GenInfo Identifier: | 121949436 |
| NCBI Gene ID: | 79733 |
| NCBI Accession: | A0AVK6.1 |
| UniProt Secondary Accession: | A0AVK6,Q2VPJ3, Q3C1U6, Q5BKY4, Q8N340, Q9H5M0, A8K9H3 |
| UniProt Related Accession: | A0AVK6 |
| Molecular Weight: | 867 |
| NCBI Full Name: | Transcription factor E2F8 |
| NCBI Synonym Full Names: | E2F transcription factor 8 |
| NCBI Official Symbol: | E2F8 |
| NCBI Official Synonym Symbols: | E2F-8 |
| NCBI Protein Information: | transcription factor E2F8; E2F family member 8 |
| UniProt Protein Name: | Transcription factor E2F8 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | E2F8 |
| UniProt Entry Name: | E2F8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using E2F8 antibody (CAB1135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
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