Cell Death Antibodies 1

E2F1 Rabbit Polyclonal Antibody (CAB2067)

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SKU:
CAB2067
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
RBP3, E2F-1, RBAP1, RBBP3, E2F1
Research Use:
Cell Death
Frequently bought together:

Description

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E2F1 Antibody (CAB2067)

The E2F1 Antibody (CAB2067) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.E2F1 is a key player in the control of cell proliferation and has been implicated in various diseases, including cancer. By binding specifically to the E2F1 protein, this antibody enables researchers to detect and analyze E2F1 expression in different cell types, providing valuable insights into its function and potential therapeutic targeting.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:E2F1 Antibody
SKU:CAB2067
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GGRL EGLT QDLR QLQE SEQQ LDHL MNIC TTQL RLLS EDTD SQRL AYVT CQDL RSIA DPAE QMVM VIKA PPET QLQA VDSS ENFQ ISLK SKQG PIDV FLCP EE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:20 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RBP3, E2F-1, RBAP1, RBBP3, E2F1
Positive Sample:HeLa, A-431, HT-29
Cellular Localization:Nucleus.
Calculated MW:47kDa
Observed MW:60kDa

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Purification MethodAffinity purification
Gene ID1869
RRIDAB_2764090
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using E2F1 Rabbit pAb (CAB2067) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of C6 cells using E2F1 Rabbit pAb (CAB2067) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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