Cell Death Antibodies 1

Anti-E2F1 Antibody (CAB16720)

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SKU:
CAB16720
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-E2F1 Antibody
Antibody SKU:CAB16720
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human E2F1
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:20 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:mouse brain, rat brain, rat testis
Immunogen:A synthetic peptide of human E2F1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1869
Uniprot:Q01094
Cellular Location:Nucleus
Calculated MW:46kDa
Observed MW:70kDa
Synonyms:E2F-1, RBAP1, RBBP3, RBP3, E2F1
Background:The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
UniProt Protein Function:E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: mitochondrion; nuclear chromatin; nucleoplasm; nucleus; Rb-E2F complex

Molecular Function:DNA binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: anoikis; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; forebrain development; mRNA stabilization; negative regulation of DNA binding; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of fibroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; spermatogenesis; transcription, DNA-dependent

NCBI Summary:The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]
UniProt Code:Q01094
NCBI GenInfo Identifier:400928
NCBI Gene ID:1869
NCBI Accession:Q01094.1
UniProt Secondary Accession:Q01094,Q13143, Q92768,
UniProt Related Accession:Q01094
Molecular Weight:46,920 Da
NCBI Full Name:Transcription factor E2F1
NCBI Synonym Full Names:E2F transcription factor 1
NCBI Official Symbol:E2F1  
NCBI Official Synonym Symbols:RBP3; E2F-1; RBAP1; RBBP3  
NCBI Protein Information:transcription factor E2F1
UniProt Protein Name:Transcription factor E2F1
UniProt Synonym Protein Names:PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Protein Family:Transcription factor
UniProt Gene Name:E2F1  
UniProt Entry Name:E2F1_HUMAN
Anti-E2F1 Antibody (CAB16720)Western blot analysis of extracts of various cell lines, using E2F1 antibody (CAB16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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