Metabolism Antibodies 2

DUT Rabbit Polyclonal Antibody (CAB2901)

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SKU:
CAB2901
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
BMFDMS, dUTPase, DUT
Research Use:
Metabolism
Frequently bought together:

Description

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DUT Antibody (CAB2901)

The DUT Antibody (CAB2901) is a high-quality antibody developed for reliable detection and analysis of target proteins. DUT is an essential enzyme involved in DNA metabolism, specifically in the prevention of uracil incorporation into DNA. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for Western blot applications. By binding to the DUT protein, researchers are able to detect and analyze its expression in various cell types, providing insights into its role in DNA repair and maintenance.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DUT Antibody
SKU:CAB2901
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PAPG PETP AISP SKRA RPAE VGGM QLRF ARLS EHAT APTR GSAR AAGY DLYS AYDY TIPP MEKA VVKT DIQI ALPS GCYG RVAP RSGL AAKH FIDV GAGV IDED YRGN VGVV LFNF GKEK FEVK KGDR IAQL ICER IFYP EIEE VQAL DDTE RGSG GFGS TGKN
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BMFDMS, dUTPase, DUT
Positive Sample:LO2, Jurkat, HeLa
Cellular Localization:Mitochondrion, Nucleus.
Calculated MW:27kDa
Observed MW:24kDa

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Purification MethodAffinity purification
Gene ID1854
RRIDAB_2764721
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DUT Rabbit pAb (CAB2901) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat spleen using DUT Rabbit pAb (CAB2901) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human appendix using DUT Rabbit pAb (CAB2901) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using DUT Rabbit pAb (CAB2901) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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