Antibody Name:	Anti-DNA polymerase eta Antibody
Antibody SKU:	CAB1833
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human DNA polymerase eta (NP_006493.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, DU145, Raji, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human DNA polymerase eta (NP_006493.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QVEQ RQNP HLRN KPCA VVQY KSWK GGGI IAVS YEAR AFGV TRSM WADD AKKL CPDL LLAQ VRES RGKA NLTK YREA SVEV MEIM SRFA VIER ASID EAYV DLTS AVQE RLQK LQGQ PISA DLLP STYI EGLP QGPT TAEE TVQK EGMR KQGL FQWL DSLQ IDNL TSPD LQLT VGAV IVEE MRAA IERE TGFQ CSAG ISHN KVLA KLAC GLNK PNRQ TLVS HGSV PQLF SQMP IRKI RSLG GKLG ASVI EILG IEYM GELT QFTE SQLQ SHFG EKNG SWLY AMCR GIEH DPVK PRQL PKTI G
Gene ID:	5429
Uniprot:	Q9Y253
Cellular Location:	Nucleus
Calculated MW:	46kDa/78kDa
Observed MW:	70kDa

Synonyms:

POLH, RAD30, RAD30A, XP-V, XPV

Background:

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; EC 2.7.7.7; DNA repair, damage Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding; metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity Biological Process: regulation of DNA repair; DNA synthesis during DNA repair; postreplication repair; pyrimidine dimer repair; DNA repair; DNA replication; response to UV-C Disease: Xeroderma Pigmentosum, Variant Type
NCBI Summary:	This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:	Q9Y253
NCBI GenInfo Identifier:	59798441
NCBI Gene ID:	5429
NCBI Accession:	Q9Y253.1
UniProt Related Accession:	Q9Y253
Molecular Weight:
NCBI Full Name:	DNA polymerase eta
NCBI Synonym Full Names:	DNA polymerase eta
NCBI Official Symbol:	POLH
NCBI Official Synonym Symbols:	XPV; XP-V; RAD30; RAD30A
NCBI Protein Information:	DNA polymerase eta
UniProt Protein Name:	DNA polymerase eta
UniProt Synonym Protein Names:	RAD30 homolog A; Xeroderma pigmentosum variant type protein
Protein Family:	DNA polymerase
UniProt Gene Name:	POLH
UniProt Entry Name:	POLH_HUMAN

	Western blot analysis of extracts of various cell lines, using DNA polymerase eta antibody (CAB1833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U-2 OS cells using DNA polymerase eta Rabbit pAb (CAB1833) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.