Immunology Antibodies 2

DHX36 Rabbit Polyclonal Antibody (CAB5191)

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SKU:
CAB5191
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
G4R1, RHAU, DDX36, MLEL1, DHX36
Research Use:
Immunology
Frequently bought together:

Description

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DHX36 Antibody (CAB5191)

The DHX36 Antibody (CAB5191) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.DHX36, also known as RNA helicase DHX36, is involved in numerous biological processes, including RNA processing, splicing, and innate immune response regulation. The DHX36 Polyclonal Antibody binds specifically to the DHX36 protein, allowing for accurate detection and analysis in various cell types.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DHX36 Antibody
SKU:CAB5191
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSYD YHQN WGRD GGPR SSGG GYGG GPAG GHGG NRGS GGGG GGGG GGRG GRGR HPGH LKGR EIGM WYAK KQGQ KNKE AERQ ERAV VHMD ERRE EQIV QLLN SVQA KNDK ESEA QISW FAPE DHGY GTEV STKN TPCS ENKL DIQE KKLI NQEK KMFR IRNR SYID RDSE YLLQ ENEP DGTL DQKL LEDL QKKK NDLR YIEM QHFR EKLP SYGM QKEL VNLI DNHQ VTVI SGET GCGK TTQV TQFI LDNY IERG KGSA CRIV
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:200 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:G4R1, RHAU, DDX36, MLEL1, DHX36
Positive Sample:Mouse brain, Mouse liver, Mouse kidney, Rat brain
Cellular Localization:Chromosome, Cytoplasm, Nucleus, Telomere.
Calculated MW:115kDa
Observed MW:114kDa

This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID170506
RRIDAB_2766051
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DHX36 Rabbit pAb (CAB5191) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunoprecipitation analysis of 200 μg extracts of MCF7 cells using 3 μg DHX36 antibody (CAB5191). Western blot was performed from the immunoprecipitate using DHX36 antibody (CAB5191) at a dilution of 1:1000.
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