Cell Biology Antibodies 4

DGKA Rabbit Polyclonal Antibody (CAB13969)

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SKU:
CAB13969
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
DAGK, DAGK1, DGK-alpha, DGKA
Research Use:
Cell Biology
Frequently bought together:

Description

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DGKA Antibody (CAB13969)

The DGKA Antibody (CAB13969) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been extensively validated for use in Western blot applications.DGKA, also known as diacylglycerol kinase alpha, plays a crucial role in cellular processes such as lipid signaling, membrane trafficking, and cell growth. Dysregulation of DGKA has been implicated in various diseases, including cancer, diabetes, and neurological disorders.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DGKA Antibody
SKU:CAB13969
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAKE RGLI SPSD FAQL QKYM EYST KKVS DVLK LFED GEMA KYVQ GDAI GYEG FQQF LKIY LEVD NVPR HLSL ALFQ SFET GHCL NETN VTKD VVCL NDVS CYFS LLEG GRPE DKLE FTFK LYDT DRNG ILDS SEVD KIIL QMMR VAEY LDWD VSEL RPIL QEMM KEID YDGS GSVS QAEW VRAG ATTV PLLV LLGL EMTL
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:5000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DAGK, DAGK1, DGK-alpha, DGKA
Positive Sample:HeLa, Jurkat, Molt-4
Cellular Localization:Cytosol, Plasma Membrane.
Calculated MW:83kDa
Observed MW:80kDa

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.

Purification MethodAffinity purification
Gene ID1606
RRIDAB_2760823
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DGKA Rabbit pAb (CAB13969) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
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