Cell Biology Antibodies 10

DGAT1 Rabbit Polyclonal Antibody (CAB6857)

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SKU:
CAB6857
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ARAT, DGAT, ARGP1, DIAR7, DGAT1
Research Use:
Cell Biology
Frequently bought together:

Description

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DGAT1 Antibody (CAB6857)

The DGAT1 Antibody (CAB6857) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the DGAT1 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in metabolic disorders, obesity, and lipid biology research.DGAT1, also known as diacylglycerol O-acyltransferase 1, is involved in the synthesis of triglycerides, which are essential for storing and transporting energy in the body.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DGAT1 Antibody
SKU:CAB6857
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:HTIL FLKL FSYR DVNS WCRR ARAK AASA GKKA SSAA APHT VSYP DNLT YRDL YYFL FAPT LCYE LNFP RSPR IRKR FLLR RILE MLFF TQLQ VGLI QQWM
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ARAT, DGAT, ARGP1, DIAR7, DGAT1
Positive Sample:HepG2, A-431, HL-60, Mouse liver
Cellular Localization:Endoplasmic Reticulum Membrane, Multi-Pass Membrane Protein.
Calculated MW:55kDa
Observed MW:50kDa

This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.

Purification MethodAffinity purification
Gene ID8694
RRIDAB_2767419
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DGAT1 Rabbit pAb (CAB6857) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from Mouse liver, using DGAT1 Rabbit pAb (CAB6857) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using DGAT1 Rabbit pAb (CAB6857) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using DGAT1 Rabbit pAb (CAB6857) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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