Anti-DENND4C Antibody [3E8]

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SKU:
STMA00216
Antibody Type:
Monoclonal
Applications:
WB
Applications:
ICC/IF
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
IgG1
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (STMA00216). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (STMA00216) at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI. Courtesy of: Dario Alessi Lab, University of Dundee.
  • Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (STMA00216). Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (STMA00216). Three amounts of STMA00216 (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C.
  • Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (STMA00216). Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (STMA00216) at 1:1000. Courtesy of: Dario Alessi Lab, University of Dundee.
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Description

DENND4C Antibody [3E8]

DENND4C Antibody [3E8] is a mouse monoclonal antibody directed against DENND4C, offered by Assay Genie for research applications. Reported applications include WB, ICC/IF and IP. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.

DENND4C is a guanine nucleotide exchange factor (GEF) for Rab10, a small GTPase involved in vesicle trafficking and membrane recycling. DENND4C regulates GLUT4 translocation and interacts with the retromer complex, linking it to endosomal sorting and cellular metabolism. Mutations in DENND4C have been associated with neurodevelopmental syndromes such as Alkuraya-Kucinskas and Koolen-De Vries, suggesting a broader role in brain development and function. Its involvement in membrane trafficking pathways positions DENND4C as a candidate for investigation in neurodegenerative diseases characterized by endolysosomal dysfunction. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:DENND4C Antibody [3E8]
Product SKU:STMA00216
Size:100 µg
Target:DENND4C
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:3E8
Research Areas:Alzheimer's Disease, Cell Signaling, Golgi Proteins, Membrane Trafficking Proteins, Neurodegeneration, Neuroscience, Parkinson's Disease, Protein Trafficking, Growth Factors
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, ICC/IF, IP
Antibody Isotype:IgG1
Purification:Protein G Purified
Concentration:1mg/ml
Reactivity:Human, Mouse, Rat
Recommended Dilution:WB (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~213 kDa.
Guarantee:12 months from date of dispatch
Immunogen:Full Length Human Recombinant Dennd4c protein
Immunogen Species:Human
Accession Number:NM_017925
Gene ID:55667
Swiss-Prot:Q5VZ89
Cellular Localization:Cell membrane | Cytoplasm | Cytoplasmic vesicle | Membrane
Tissue Specificity:Widely expressed
Alternative Names:DENN domain-containing protein 4C
References:1. Sano, H. et al. (2011). J Biol Chem. 286(19):16541-5. 2. https://www.genecards.org/cgi-bin/carddisp.pl?gene=DENND4C 3. McMillan, K. et al. (2016). J Cell Biol. 214(4):4389–400
Note:For research use only.
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