Cell Death Antibodies 1

Anti-DDX47 Antibody (CAB10359)

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SKU:
CAB10359
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-DDX47 Antibody
Antibody SKU:CAB10359
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SKOV3, LO2, HeLa, Mouse testis, Mouse lung, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TEVD KILK VIPR DRKT FLFS ATMT KKVQ KLQR AALK NPVK CAVS SKYQ TVEK LQQY YIFI PSKF KDTY LVYI LNEL AGNS FMIF CSTC NNTQ RTAL LLRN LGFT AIPL HGQM SQSK RLGS LNKF KAKA RSIL LATD VASR GLDI PHVD VVVN FDIP THSK DYIH RVGR TARA GRSG KAIT FVTQ YDVE LFQR IEHL IGKK LPGF PTQD DEVM MLTE RVAE AQRF ARME LREH GEKK KRSR EDAG DNDD TEGA IGVR NKVA GGKM KKRK GR
Gene ID:51202
Uniprot:Q9H0S4
Cellular Location:Nucleus, nucleolus
Calculated MW:45kDa/50kDa
Observed MW:50kDa
Synonyms:DDX47, E4-DBP, HQ0256, MSTP162, RRP3
Background:This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:DDX47: a protein of the DEAD box helicase family. Unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
UniProt Protein Details:

Protein type:EC 3.6.4.13; Helicase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: membrane; nucleolus; nucleoplasm

Molecular Function:ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: induction of apoptosis via death domain receptors; RNA secondary structure unwinding; RNA splicing; rRNA processing

NCBI Summary:This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H0S4
NCBI GenInfo Identifier:52782792
NCBI Gene ID:51202
NCBI Accession:Q9H0S4.1
UniProt Secondary Accession:Q9H0S4,Q96GM0, Q96NV8, Q9UI98, B3KXP4, G5E955,
UniProt Related Accession:Q9H0S4
Molecular Weight:51kDa
NCBI Full Name:Probable ATP-dependent RNA helicase DDX47
NCBI Synonym Full Names:DEAD-box helicase 47
NCBI Official Symbol:DDX47  
NCBI Official Synonym Symbols:RRP3; E4-DBP; HQ0256; MSTP162  
NCBI Protein Information:probable ATP-dependent RNA helicase DDX47
UniProt Protein Name:Probable ATP-dependent RNA helicase DDX47
UniProt Synonym Protein Names:DEAD box protein 47
Protein Family:Probable ATP-dependent RNA helicase
UniProt Gene Name:DDX47  
Anti-DDX47 Antibody (CAB10359)Western blot analysis of extracts of various cell lines, using DDX47 antibody (CAB10359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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