Antibody Name:	Anti-DDX4 Antibody
Antibody SKU:	CAB15624
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX4 (NP_077726.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX4 (NP_077726.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDE DWEA EINP HMSS YVPI FEKD RYSG ENGD NFNR TPAS SSEM DDGP SRRD HFMK SGFA SGRN FGNR DAGE CNKR DNTS TMGG FGVG KSFG NRGF SNSR FEDG DSSG FWRE SSND CEDN PTRN RGFS KRGG YRDG NNSE ASGP YRRG GRGS FRGC RGGF GLGS PNND LDPD ECMQ RTGG LFGS RRPV LSGT GNGD TSQS RSGS GSER GGYK GLNE EVIT GSGK NSWK SEAE GGES SDTQ GPKV TYIP PPPP EDED SIFA HYQT GINF DKYD TILV EVSG HDAP PAIL TFEE ANLC QTLN
Gene ID:	54514
Uniprot:	Q9NQI0
Cellular Location:	Cytoplasm, perinuclear region
Calculated MW:	63kDa/75kDa/77kDa/79kDa
Observed MW:	79KDa

Synonyms:

DDX4, VASA

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	DDX4: May play a role in germ cell development. May play a role in sperm motility. Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Belongs to the DEAD box helicase family. DDX4/VASA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.4.13; Helicase Chromosomal Location of Human Ortholog: 5p15.2-p13.1 Cellular Component: cytoplasm; perinuclear region of cytoplasm Molecular Function:ATP-dependent RNA helicase activity Biological Process: DNA methylation during gametogenesis; male meiosis; RNA secondary structure unwinding; sperm motility; spermatogenesis
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	Q9NQI0
NCBI GenInfo Identifier:	20138033
NCBI Gene ID:	54514
NCBI Accession:	Q9NQI0.2
UniProt Secondary Accession:	Q9NQI0,Q5M7Z3, Q86VX0, Q9NT92, Q9NYB1, A8K8Q2, B3KSF4 D6RDK4, E9PCD8,
UniProt Related Accession:	Q9NQI0
Molecular Weight:	77,056 Da
NCBI Full Name:	Probable ATP-dependent RNA helicase DDX4
NCBI Synonym Full Names:	DEAD-box helicase 4
NCBI Official Symbol:	DDX4
NCBI Official Synonym Symbols:	VASA
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX4
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX4
UniProt Synonym Protein Names:	DEAD box protein 4; Vasa homolog
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX4
UniProt Entry Name:	DDX4_HUMAN

	Western blot analysis of extracts of Mouse testis, using DDX4 antibody (CAB15624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.