Cell Biology Antibodies 6

DDX24 Rabbit Polyclonal Antibody (CAB15481)

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SKU:
CAB15481
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
DDX24
Research Use:
Cell Biology
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Description

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DDX24 Antibody (CAB15481)

The DDX24 Antibody (CAB15481) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the DDX24 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and gene regulation.DDX24 plays a crucial role in various cellular processes, including RNA splicing, translation, and mRNA decay, making it a key player in gene expression control.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DDX24 Antibody
SKU:CAB15481
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LTLV HQAP ARIL HKKH TKKM DKTA KLDL LMQK IGMR GKPK VIDL TRNE ATVE TLTE TKIH CETD EKDF YLYY FLMQ YPGR SLVF ANSI SCIK RLSG LLKV LDIM PLTL HACM HQKQ RLRN LEQF ARLE DCVL LATD VAAR GLDI PKVQ HVIH YQVP RTSE IYVH RSGR TARA TNEG LSLM LIGP EDVI NFKK IYKT LKKD EDIP LFPV QTKY MDVV KERI RLAR QIEK SEYR NFQA CLHN SWIE QAAA ALEI ELEE DMYK GGKA DQQE ERRR QKQM KVLK KELR HLLS QPLF TESQ KTKY PTQS GKPP LLVS APSK SESA LSCL SKQK KKKT KKPK EPQP EQPQ PSTS AN
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:200 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DDX24
Positive Sample:HT-29, LO2, Jurkat, HeLa
Cellular Localization:Nucleolus.
Calculated MW:96kDa
Observed MW:120kDa

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level.

Purification MethodAffinity purification
Gene ID57062
RRIDAB_2762880
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DDX24 Rabbit pAb (CAB15481) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of C6 cells using DDX24 Rabbit pAb (CAB15481) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using DDX24 Rabbit pAb (CAB15481) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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