ChIP Antibodies

Anti-DDX11 Antibody (CAB7666)

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SKU:
CAB7666
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DDX11 Antibody
Antibody SKU:CAB7666
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX11 (NP_689651.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HT-29, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX11 (NP_689651.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANE TQKV GAIH FPFP FTPY SIQE DFMA ELYR VLEA GKIG IFES PTGT GKSL SLIC GALS WLRD FEQK KREE EARL LETG TGPL HDEK DESL CLSS SCEG AAGT PRPA GEPA WVTQ FVQK KEER DLVD RLKA EQAR RKQR EERL QQLQ HRVQ LKYA AKRL RQEE EERE NLLR LSRE MLET GPEA ERLE QLES GEEE LVLA EYES DEEK KVAS RVDE DEDD
Gene ID:1663
Uniprot:Q96FC9
Cellular Location:Nucleus, nucleolus
Calculated MW:32kDa/96kDa/98kDa/101kDa/108kDa
Observed MW:108kDa
Synonyms:DDX11, CHL1, CHLR1, KRG2, WABS
Background:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.4.13; Nucleolus; Helicase

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: midbody; nuclear chromatin; nucleolus; nucleoplasm; nucleus; spindle pole

Molecular Function:DNA-dependent ATPase activity; double-stranded DNA binding; helicase activity; protein binding; single-stranded DNA binding

Biological Process: sister chromatid cohesion

Disease: Warsaw Breakage Syndrome

NCBI Summary:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q96FC9
NCBI GenInfo Identifier:74731686
NCBI Gene ID:1663
NCBI Accession:Q96FC9.1
UniProt Secondary Accession:Q96FC9,Q13333, Q86VQ4, Q86W62, Q92498, Q92770, Q92998 Q92999,
UniProt Related Accession:Q96FC9
Molecular Weight:32,951 Da
NCBI Full Name:Probable ATP-dependent DNA helicase DDX11
NCBI Synonym Full Names:DEAD/H-box helicase 11
NCBI Official Symbol:DDX11  
NCBI Official Synonym Symbols:CHL1; KRG2; WABS; CHLR1  
NCBI Protein Information:probable ATP-dependent DNA helicase DDX11
UniProt Protein Name:Probable ATP-dependent DNA helicase DDX11
UniProt Synonym Protein Names:CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2
Protein Family:ATP-dependent DNA helicase
UniProt Gene Name:DDX11  
UniProt Entry Name:DDX11_HUMAN
Anti-DDX11 Antibody (CAB7666)Western blot analysis of extracts of various cell lines, using DDX11 antibody (CAB7666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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