KO Validated Antibodies 1

[KO Validated] DDIT4 Rabbit Polyclonal Antibody (CAB8086)

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SKU:
CAB8086
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Synonyms:
Dig2, REDD1, REDD-1, T4
Research Use:
Immunology
Frequently bought together:

Description

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[KO Validated] DDIT4 Antibody (CAB8086)

The [KO Validated] DDIT4 Antibody (CAB8086) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced against DDIT4 in rabbits, is specifically validated for knockout (KO) studies and demonstrated to have high specificity and sensitivity in Western blot applications.DDIT4, also known as REDD1, is a critical factor in the cellular response to nutrient deprivation, hypoxia, and other stress conditions. By binding to DDIT4, this polyclonal antibody allows for precise detection and quantification of DDIT4 expression in various cell types, enabling researchers to explore its role in cellular metabolism, signal transduction, and disease processes.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] DDIT4 Antibody
SKU:CAB8086
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPSL WDRF SSSS TSSS PSSL PRTP TPDR PPRS AWGS ATRE EGFD RSTS LESS DCES LDSS NSGF GPEE DTAY LDGV SLPD FELL SDPE DEHL CANL MQLL QESL AQAR LGSR RPAR LLMP SQLV SQVG KELL RLAY SEPC GLRG ALLD VCVE QGKS CHSV GQLA LDPS LVPT FQLT LVLR LDSR LWPK IQGL FSSA NSPF LPGF SQSL TLST GFRV IKKK LYSS EQLL IEEC
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Dig2, REDD1, REDD-1, T4
Positive Sample:A-549, DU145, U-251MG
Cellular Localization:Cytoplasm, Mitochondrion, Cytosol.
Calculated MW:25kDa
Observed MW:32kDa

Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.

Purification MethodAffinity purification
Gene ID54541
RRIDAB_2863566
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and DDIT4 knockout (KO) HeLa cells, using [KO Validated] DDIT4 Rabbit pAb (CAB8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of various lysates using [KO Validated] DDIT4 Rabbit pAb (CAB8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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