Cell Biology Antibodies 10

DDAH2 Rabbit Polyclonal Antibody (CAB6457)

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SKU:
CAB6457
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
G6a, DDAH, NG30, DDAHII, HEL-S-277, DDAH2
Research Use:
Cell Biology
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Description

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DDAH2 Antibody (CAB6457)

The DDAH2 Antibody (CAB6457) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By targeting the DDAH2 protein, this antibody enables precise detection and analysis in a variety of cell types, facilitating research in cardiovascular disease, hypertension, and other conditions related to nitric oxide dysregulation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DDAH2 Antibody
SKU:CAB6457
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGTP GEGL GRCS HALI RGVP ESLA SGEG AGAG LPAL DLAK AQRE HGVL GGKL RQRL GLQL LELP PEES LPLG PLLG DTAV IQGD TALI TRPW SPAR RPEV DGVR KALQ DLGL RIVE IGDE NATL DGTD VLFT GREF FVGL SKWT NHRG AEIV ADTF RDFA VSTV PVSG PSHL RGLC GMGG PRTV VAGS SDAA QKAV RAMA VLTD HPYA SLTL PDDA AADC LFLR PGLP GVPP FLLH RGGG DLPN SQEA LQKL SDVT LVPV SCSE LEKA GAGL SSLC LVLS TRPH S
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:G6a, DDAH, NG30, DDAHII, HEL-S-277, DDAH2
Positive Sample:SW620, BT-474, MCF7, Mouse lung, Rat brain
Cellular Localization:Cytoplasm, Mitochondrion.
Calculated MW:30kDa
Observed MW:30kDa

This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

Purification MethodAffinity purification
Gene ID23564
RRIDAB_2767059
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DDAH2 Rabbit pAb (CAB6457) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of U2OS cells using DDAH2 Rabbit pAb (CAB6457). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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