Cell Biology Antibodies 1

DDAH1 Rabbit Polyclonal Antibody (CAB10295)

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SKU:
CAB10295
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
DDAH, DDAHI, DDAH-1, HEL-S-16, DDAH1
Research Use:
Cell Biology
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Description

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DDAH1 Antibody (CAB10295)

The DDAH1 Antibody (CAB10295) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for applications such as Western blot analysis.DDAH1 is a critical player in the nitric oxide pathway, with implications in various physiological processes including vascular tone regulation and cardiovascular function. Dysregulation of DDAH1 has been linked to conditions such as hypertension and cardiovascular disease, underscoring its importance in human health.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DDAH1 Antibody
SKU:CAB10295
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAGL GHPA AFGR ATHA VVRA LPES LGQH ALRS AKGE EVDV ARAE RQHQ LYVG VLGS KLGL QVVE LPAD ESLP DCVF VEDV AVVC EETA LITR PGAP SRRK EVDM MKEA LEKL QLNI VEMK DENA TLDG GDVL FTGR EFFV GLSK RTNQ RGAE ILAD TFKD YAVS TVPV ADGL HLKS FCSM AGPN LIAI GSSE SAQK ALKI MQQM SDHR YDKL TVPD DIAA NCIY LNIP NKGH VLLH RTPE EYPE SAKV YEKL KDHM LIPV SMSE LEKV DGLL TCCS VLIN KKVD S
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DDAH, DDAHI, DDAH-1, HEL-S-16, DDAH1
Positive Sample:SW480, 22Rv1, HT-29, 293T, SGC-7901, Mouse brain, Mouse kidney, Rat brain
Cellular Localization:Cytosol, Extracellular Exosome.
Calculated MW:31kDa
Observed MW:37kDa

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Purification MethodAffinity purification
Gene ID23576
RRIDAB_2757838
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DDAH1 Rabbit pAb (CAB10295) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using DDAH1 Rabbit pAb (CAB10295) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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