Epigenetics & Nuclear Signaling Antibodies 4
Anti-DCP2 Antibody (CAB8282)
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- SKU:
- CAB8282
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | DCP2 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8282 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | METK RVEI PGSV LDDL CSRF ILHI PSEE RDNA IRVC FQIE LAHW FYLD FYMQ NTPG LPQC GIRD FAKA VFSH CPFL LPQG EDVE KVLD EWKE YKMG VPTY GAII LDET LENV LLVQ GYLA KSGW GFPK GKVN KEEA PHDC AARE VFEE TGFD IKDY ICKD DYIE LRIN DQLA RLYI IPGI PKDT KFNP KTRR EIRN IEWF SIEK LPCH RNDM TPKS KLGL APNK FFMA IPFI RPLR DWLS |
| Gene ID: | 167227 |
| Uniprot: | Q8IU60 |
| Cellular Location: | Cytoplasm, Nucleus, P-body |
| Calculated MW: | 44kDa/48kDa |
| Observed MW: | 50kDa |
| Synonyms: | DCP2, NUDT20 |
| Background: | The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| UniProt Protein Function: | DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.1.62; Hydrolase; RNA processing Chromosomal Location of Human Ortholog: 5q22.2 Cellular Component: cell junction; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:exoribonuclease activity, producing 5'-phosphomonoesters; m7G(5')pppN diphosphatase activity; protein binding Biological Process: deadenylation-dependent decapping; mRNA catabolic process; mRNA catabolic process, nonsense-mediated decay; regulation of mRNA stability |
| NCBI Summary: | The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011] |
| UniProt Code: | Q8IU60 |
| NCBI GenInfo Identifier: | 269849560 |
| NCBI Gene ID: | 167227 |
| NCBI Accession: | Q8IU60.2 |
| UniProt Secondary Accession: | Q8IU60,Q6P2D4, Q7Z5W5, Q8NBG5, C9J778, |
| UniProt Related Accession: | Q8IU60 |
| Molecular Weight: | 44,443 Da |
| NCBI Full Name: | m7GpppN-mRNA hydrolase |
| NCBI Synonym Full Names: | decapping mRNA 2 |
| NCBI Official Symbol: | DCP2 |
| NCBI Official Synonym Symbols: | NUDT20 |
| NCBI Protein Information: | m7GpppN-mRNA hydrolase |
| UniProt Protein Name: | m7GpppN-mRNA hydrolase |
| UniProt Synonym Protein Names: | Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20; mRNA-decapping enzyme 2; hDpc |
| Protein Family: | mRNA-decapping enzyme |
| UniProt Gene Name: | DCP2 |
| UniProt Entry Name: | DCP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DCP2 antibody (CAB8282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using DCP2 antibody (CAB8282) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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