The DBF4 Monoclonal Antibody (CAB19766) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.DBF4 is essential for the initiation of DNA replication and plays a critical role in ensuring the accurate duplication of genetic material during cell division. Dysregulation of DBF4 has been implicated in various types of cancer and genetic disorders, making it an important target for research in oncology and developmental biology.By targeting the DBF4 protein, this antibody enables researchers to study its expression and function in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DBF4 Monoclonal Antibody
SKU:
CAB19766
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2293
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASK, CHIF, DBF4A, ZDBF1, DBF4
Positive Sample:
PC-3, Jurkat
Cellular Localization:
Nuclear Body, Nucleoplasm, Nucleus.
Calculated MW:
77kDa
Observed MW:
77kDa
Predicted to enable protein serine/threonine kinase activator activity. Predicted to be involved in positive regulation of nuclear cell cycle DNA replication and regulation of cell cycle phase transition. Located in nuclear body.
Purification Method
Affinity purification
Gene ID
10926
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DBF4 Rabbit mAb (CAB19766) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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