Metabolism Antibodies 2

DARS2 Rabbit Polyclonal Antibody (CAB7813)

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SKU:
CAB7813
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse
Synonyms:
LBSL, ASPRS, mtAspRS, MT-ASPRS, DARS2
Research Use:
Metabolism
Frequently bought together:

Description

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DARS2 Antibody (CAB7813)

The DARS2 Antibody (CAB7813) is a high-quality antibody developed for reliable detection and analysis of target proteins. DARS2 is an important enzyme involved in mitochondrial function and is linked to various diseases, including leukoencephalopathy and hypertrophic cardiomyopathy. The antibody, produced in rabbits, is suitable for use in Western blot applications and is highly reactive with human samples.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:DARS2 Antibody
SKU:CAB7813
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ANRN WNSP VANF IMES QRLE LIRL METQ EEDV VLLT AGEH NKAC SLLG KLRL ECAD LLET RGVV LRDP TLFS FLWV VDFP LFLP KEEN PREL ESAH HPFT APHP SDIH LLYT EPKK ARSQ HYDL VLNG NEIG GGSI RIHN AELQ RYIL ATLL KEDV KMLS HLLQ ALDY GAPP HGGI ALGL DRLI CLVT GSPS IRDV IAFP KSFR GHDL MSNT PDSV PPEE LKPY HIRV SKPT DSKA ERAH
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LBSL, ASPRS, mtAspRS, MT-ASPRS, DARS2
Positive Sample:NCI-H460, U-251MG, MCF7, BT-474, Mouse liver, Mouse heart
Cellular Localization:Mitochondrion Matrix.
Calculated MW:74kDa
Observed MW:74kDa

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).

Purification MethodAffinity purification
Gene ID55157
RRIDAB_2769114
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DARS2 Rabbit pAb (CAB7813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of various lysates using DARS2 Rabbit pAb (CAB7813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using DARS2 Rabbit pAb (CAB7813) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using DARS2 Rabbit pAb (CAB7813) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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