CYPOR Rabbit Monoclonal Antibody (CAB5032)

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SKU:
CAB5032
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CPR, CYPOR, P450R
Research Use:
Cell Biology
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Description

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CYPOR Monoclonal Antibody (CAB5032)

The CYPOR Monoclonal Antibody (CAB5032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the CYPOR protein, this antibody enables accurate detection and analysis in a variety of cell types, making it invaluable for investigations in pharmacology and toxicology.CYPOR plays a crucial role in the activation of various drugs and xenobiotics by facilitating their metabolism in the liver.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CYPOR Monoclonal Antibody
SKU:CAB5032
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1981
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KDAH RYGM RGMS ADPE EYDL ADLS SLPE IDNA LVVF CMAT YGEG DPTD NAQD FYDW LQET DVDL SGVK FAVF GLGN KTYE HFNA MGKY VDKR LEQL GAQR I
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CPR, CYPOR, P450R
Positive Sample:Hep G2, Mouse lung, Mouse liver, Rat liver
Cellular Localization:Endoplasmic Reticulum Membrane, Peripheral Membrane Protein.
Calculated MW:77kDa
Observed MW:77kDa

This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase.

Purification MethodAffinity purification
Gene ID5447
RRIDAB_2863422
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CYPOR Rabbit mAb (CAB5032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CYPOR Rabbit mAb (CAB5032) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using CYPOR Rabbit mAb (CAB5032) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver tissue using CYPOR Rabbit mAb (CAB5032) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using CYPOR Rabbit mAb (CAB5032) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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