Epigenetics & Nuclear Signaling Antibodies 2
Anti-CNOT1 Antibody (CAB5969)
- SKU:
- CAB5969
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CNOT1 Antibody |
| Antibody SKU: | CAB5969 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2137-2376 of human CNOT1 (NP_057368.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, 293T, LO2, A-549, HeLa, mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2137-2376 of human CNOT1 (NP_057368.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PRNM RLPD PFTP NLKV DMLS EINI APRI LTNF TGVM PPQF KKDL DSYL KTRS PVTF LSDL RSNL QVSN EPGN RYNL QLIN ALVL YVGT QAIA HIHN KGST PSMS TITH SAHM DIFQ NLAV DLDT EGRY LFLN AIAN QLRY PNSH THYF SCTM LYLF AEAN TEAI QEQI TRVL LERL IVNR PHPW GLLI TFIE LIKN PAFK FWNH EFVH CAPE IEKL FQSV AQCC MGQK QAQQ VMEG TGAS |
| Gene ID: | 23019 |
| Uniprot: | A5YKK6 |
| Cellular Location: | Cytoplasm, Nucleus, P-body |
| Calculated MW: | 173kDa/241kDa/266kDa |
| Observed MW: | 267kDa |
| Synonyms: | CNOT1, AD-005, CDC39, NOT1, NOT1H |
| Background: |
| UniProt Protein Function: | NOT1: represses ligand-dependent transcriptional activation by oestrogen receptor (ER) alpha and several RXR heterodimers. Interacts with the ligand-binding domain of estrogen receptor-alpha in a hormone-dependent fashion. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor; RNA-binding Chromosomal Location of Human Ortholog: 16q21 Cellular Component: nucleoplasm; peroxisomal membrane; extracellular space; membrane; cytosol; CCR4-NOT complex Molecular Function:protein domain specific binding; protein binding; poly(A)-specific ribonuclease activity; retinoic acid receptor binding; estrogen receptor binding; protein complex scaffold Biological Process: poly(A) tail shortening; negative regulation of retinoic acid receptor signaling pathway; transcription, DNA-dependent; negative regulation of translation; multicellular organismal development; gene expression; negative regulation of transcription from RNA polymerase II promoter; miRNA-mediated gene silencing; negative regulation of estrogen receptor signaling pathway; mRNA catabolic process, deadenylation-dependent decay |
| UniProt Code: | A5YKK6 |
| NCBI GenInfo Identifier: | 166216086 |
| NCBI Gene ID: | 23019 |
| NCBI Accession: | A5YKK6.2 |
| UniProt Secondary Accession: | A5YKK6,Q68DX7, Q7Z3K2, Q8IWB8, Q8TB53, Q9BVZ6, Q9UFR8 Q9UI27, Q9Y2L0, |
| UniProt Related Accession: | A5YKK6 |
| Molecular Weight: | 2376 |
| NCBI Full Name: | CCR4-NOT transcription complex subunit 1 |
| NCBI Synonym Full Names: | CCR4-NOT transcription complex, subunit 1 |
| NCBI Official Symbol: | CNOT1 |
| NCBI Official Synonym Symbols: | NOT1; CDC39; NOT1H; AD-005 |
| NCBI Protein Information: | CCR4-NOT transcription complex subunit 1; CCR4-associated factor 1; adrenal gland protein AD-005; negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog |
| UniProt Protein Name: | CCR4-NOT transcription complex subunit 1 |
| UniProt Synonym Protein Names: | CCR4-associated factor 1; Negative regulator of transcription subunit 1 homolog; NOT1H; hNOT1 |
| Protein Family: | CCR4-NOT transcription complex |
| UniProt Gene Name: | CNOT1 |
| UniProt Entry Name: | CNOT1_HUMAN |
 | Western blot analysis of extracts of various cell line, using CNOT1 antibody (CAB5969) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb (CAB5969) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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