Cell Biology Antibodies 1

CLUH Rabbit Polyclonal Antibody (CAB10140)

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SKU:
CAB10140
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
CLU1, CLUH
Research Use:
Cell Biology
Frequently bought together:

Description

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CLUH Antibody (CAB10140)

The CLUH Antibody (CAB10140) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is well-suited for use in Western blot applications. By specifically binding to the CLUH protein, researchers can easily detect and analyze its expression in different cell types, making it an essential tool for studies in molecular biology and mitochondrial research.CLUH, also known as cluster of differentiation 300A, is essential for maintaining cellular homeostasis by regulating RNA stability and mitochondrial function. Its role in these processes makes it a crucial target for research into diseases such as mitochondrial disorders, neurodegenerative diseases, and metabolic syndromes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:CLUH Antibody
SKU:CAB10140
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:YDLS LRFL ENAL AVST KYHG PKAL KVAL SHHL VARV YESK AEFR SALQ HEKE GYTI YKTQ LGED HEKT KESS EYLK CLTQ QAVA LQRT MNEI YRNG SSAN IPPL KFTA PSMA SVLE QLNV INGI LFIP LSQK DLEN LKAE VARR HQLQ EASR NRDR AEEP MATE PAPA GAPG DLGS QPPA AKDP SPSV QG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CLU1, CLUH
Positive Sample:HeLa, K-562, MCF7, U-251MG, LO2
Cellular Localization:Cytoplasm, Cytoplasmic Granule.
Calculated MW:147kDa
Observed MW:180kDa

Enables mRNA binding activity. Involved in intracellular distribution of mitochondria. Located in cytoplasm.

Purification MethodAffinity purification
Gene ID23277
RRIDAB_2757667
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CLUH Rabbit pAb (CAB10140) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using CLUH Rabbit pAb (CAB10140) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using CLUH Rabbit pAb (CAB10140) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using CLUH Rabbit pAb (CAB10140) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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