Cell Death Antibodies 1

CLU Rabbit Polyclonal Antibody (CAB13479)

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SKU:
CAB13479
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-40, TRPM2, TRPM-2, NA1/NA2, Clusterin
Research Use:
Cell Death
Frequently bought together:

Description

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CLU Antibody (CAB13479)

The CLU Antibody (CAB13479) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly sensitive and specific to human samples, making it ideal for use in various applications such as Western blotting and immunohistochemistry.Clusterin, also known as apolipoprotein J, is a versatile protein that has been implicated in several disease processes, including cancer, neurodegenerative disorders, and cardiovascular diseases.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CLU Antibody
SKU:CAB13479
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:FPKS RIVR SLMP FSPY EPLN FHAM FQPF LEMI HEAQ QAMD IHFH SPAF QHPP TEFI REGD DDRT VCRE IRHN STGC LRMK DQCD KCRE ILSV DCST NNPS QAKL RREL DESL QVAE RLTR KYNE LLKS YQWK MLNT SSLL EQLN EQFN WVSR LANL TQGE DQYY LRVT TVAS HTSD SDVP SGVT EVVV KLFD SDPI TVTV PVEV SRKN PKFM ETVA EKAL QEYR KKHR EE
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-40, TRPM2, TRPM-2, NA1/NA2, Clusterin
Positive Sample:HepG2, U-251MG, Human plasma, Mouse plasma
Cellular Localization:Cytoplasm, Cytoplasmic Side, Cytoplasmic Vesicle, Endoplasmic Reticulum, Microsome, Mitochondrion Membrane, Nucleus, Peripheral Membrane Protein, Secreted, Chromaffin Granule, Cytosol, Secretory Vesicle.
Calculated MW:52kDa
Observed MW:38kDa/57kDa

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.

Purification MethodAffinity purification
Gene ID1191
RRIDAB_2760341
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Clusterin Rabbit pAb (CAB13479) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Clusterin Rabbit pAb (CAB13479) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of U2OS cells using Clusterin Rabbit pAb (CAB13479). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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