Cell Biology Antibodies 1

CLDN5 Rabbit Polyclonal Antibody (CAB10207)

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SKU:
CAB10207
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
AWAL, BEC1, TMVCF, TMDVCF, CPETRL1, CLDN5
Research Use:
Cell Biology
Frequently bought together:

Description

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CLDN5 Antibody (CAB10207)

The CLDN5 Antibody (CAB10207) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets Claudin-5 in human samples and is validated for use in various applications, including immunofluorescence and immunohistochemistry.Claudin-5 is essential for maintaining the integrity of the blood-brain barrier, which is crucial for protecting the central nervous system from harmful substances and pathogens.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CLDN5 Antibody
SKU:CAB10207
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LTGG VLYL FCGL LALV PLCW FANI VVRE FYDP SVPV SQKY ELGA ALYI GWAA TALL MVGG CLLC CGAW VCTG RPDL SFPV KYSA PRRP TATG DYDK KNYV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:1000 - 1:4000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AWAL, BEC1, TMVCF, TMDVCF, CPETRL1, CLDN5
Positive Sample:Mouse lung, Rat lung
Cellular Localization:Cell Junction, Cell Membrane, Multi-Pass Membrane Protein, Tight Junction.
Calculated MW:23kDa
Observed MW:22kDa

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification MethodAffinity purification
Gene ID7122
RRIDAB_2757729
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using CLDN5 Rabbit pAb (CAB10207) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung tissue using CLDN5 Rabbit pAb (CAB10207) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung tissue using CLDN5 Rabbit pAb (CAB10207) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CLDN5 Rabbit pAb (CAB10207) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using CLDN5 Rabbit pAb (CAB10207) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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