Cell Biology Antibodies 2

Anti-CHRM1 Antibody (CAB12323)

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SKU:
CAB12323
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CHRM1 Antibody
Antibody SKU:CAB12323
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CHRM1 (NP_000729.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HepG2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CHRM1 (NP_000729.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WELG YWLC YVNS TINP MCYA LCNK AFRD TFRL LLLC RWDK RRWR KIPK RPGS VHRT PSRQ C
Gene ID:1128
Uniprot:P11229
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:50kDa/51kDa
Observed MW:65kDa
Synonyms:CHRM1, HM1, M1, M1R
Background:The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
UniProt Protein Function:mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.
UniProt Protein Details:

Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: membrane; plasma membrane; synapse

Molecular Function:G-protein coupled acetylcholine receptor activity; phosphoinositide phospholipase C activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; cell proliferation; G-protein coupled receptor protein signaling pathway; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; nervous system development; positive regulation of cell proliferation; positive regulation of ion transport; protein kinase C activation; protein modification process; regulation of vascular smooth muscle contraction; signal transduction; synaptic transmission, cholinergic

NCBI Summary:The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]
UniProt Code:P11229
NCBI GenInfo Identifier:113118
NCBI Gene ID:1128
NCBI Accession:P11229.2
UniProt Secondary Accession:P11229,Q96RH1,
UniProt Related Accession:P11229
Molecular Weight:51kDa
NCBI Full Name:Muscarinic acetylcholine receptor M1
NCBI Synonym Full Names:cholinergic receptor muscarinic 1
NCBI Official Symbol:CHRM1  
NCBI Official Synonym Symbols:M1; HM1; M1R  
NCBI Protein Information:muscarinic acetylcholine receptor M1
UniProt Protein Name:Muscarinic acetylcholine receptor M1
Protein Family:Muscarinic acetylcholine receptor
UniProt Gene Name:CHRM1  
Anti-CHRM1 Antibody (CAB12323)Western blot analysis of extracts of various cell lines, using CHRM1 antibody (CAB12323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CHRM1 Antibody (CAB12323)Immunohistochemistry of paraffin-embedded mouse brain using CHRM1 antibody (CAB12323) at dilution of 1:100 (40x lens).
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