Anti-Choline Acetyltransferase Antibody

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SKU:
STPA00074
Antibody Type:
Polyclonal
Applications:
WB
Applications:
ICC/IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (STPA00074). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (STPA00074) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT.
  • Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (STPA00074). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (STPA00074) at 1:1000 for 1 hour at RT.
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Description

Choline Acetyltransferase Antibody

Choline Acetyltransferase Antibody is a rabbit polyclonal antibody directed against Choline Acetyltransferase, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human and Mouse. Purified by Peptide Affinity.

Choline acetyltransferase (ChAT) catalyzes the synthesis of acetylcholine (ACh), a neurotransmitter essential for motor control, memory, and autonomic function. ChAT is selectively expressed in cholinergic neurons, making it a reliable marker for cholinergic system integrity. Loss of ChAT activity is a hallmark of several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and Down syndrome. Reduced ChAT expression correlates with cognitive decline and impaired synaptic transmission in affected brain regions. As a neuron-specific enzyme with a direct role in neurotransmitter synthesis, ChAT is both a diagnostic marker and a therapeutic target in disorders characterized by cholinergic dysfunction. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:Choline Acetyltransferase Antibody
Product SKU:STPA00074
Size:100 µg
Target:Choline Acetyltransferase
Host Species:Rabbit
Antibody Type:Polyclonal
Research Areas:Neuroscience, Neurotransmission, Neurotransmitter Receptors, Cell Signaling, Neurodegeneration, Alzheimer's Disease
Buffer:PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, ICC/IF
Purification:Peptide Affinity Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse
Recommended Dilution:WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
Guarantee:12 months from date of dispatch
Immunogen:Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
Immunogen Species:Human
Accession Number:NP_001136401.1
Gene ID:1103
Swiss-Prot:P28329
Cellular Localization:Cytoplasm | Cytosol | Mitochondrion | Nucleus
Alternative Names:CHAT, Choline acetyltransferase, Acetyl-CoA choline O-acetyltransferase, Choline acetylase, CLAT_HUMAN, CMS1A
References:1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.
Note:For research use only.
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