Immunology Antibodies 2

CHMP4B Rabbit Polyclonal Antibody (CAB7402)

(No reviews yet) Write a Review
SKU:
CAB7402
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
SNF7, CTPP3, Shax1, CHMP4A, SNF7-2, VPS32B, CTRCT31, Vps32-2, C20orf178, dJ553F4.4, CHMP4B
Research Use:
Immunology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

CHMP4B Antibody (CAB7402)

The CHMP4B Antibody (CAB7402) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for CHMP4B and is validated for use in various applications, including Western blotting.CHMP4B, part of the ESCRT-III complex, plays a crucial role in the final stages of cytokinesis and multivesicular body (MVB) formation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CHMP4B Antibody
SKU:CAB7402
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSVF GKLF GAGG GKAG KGGP TPQE AIQR LRDT EEML SKKQ EFLE KKIE QELT AAKK HGTK NKRA ALQA LKRK KRYE KQLA QIDG TLST IEFQ REAL ENAN TNTE VLKN MGYA AKAM KAAH DNMD IDKV DELM QDIA DQQE LAEE ISTA ISKP VGFG EEFD EDEL MAEL EELE QEEL DKNL LEIS GPET VPLP NVPS IALP SKPA KKKE EEDD DMKE LENW AGSM
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SNF7, CTPP3, Shax1, CHMP4A, SNF7-2, VPS32B, CTRCT31, Vps32-2, C20orf178, dJ553F4.4, CHMP4B
Positive Sample:Mouse spleen
Cellular Localization:Cytoplasm, Late Endosome Membrane, Peripheral Membrane Protein, Cytosol.
Calculated MW:25kDa
Observed MW:25kDa

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.

Purification MethodAffinity purification
Gene ID128866
RRIDAB_2767933
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse spleen using CHMP4B Rabbit pAb (CAB7402) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using CHMP4B Rabbit pAb (CAB7402) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products