Epigenetics & Nuclear Signaling Antibodies 1

CHD4 Rabbit Polyclonal Antibody (CAB11574)

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SKU:
CAB11574
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
CHD-4, Mi-2b, SIHIWES, Mi2-BETA, CHD4
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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CHD4 Antibody (CAB11574)

The CHD4 Antibody (CAB11574) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards CHD4 protein in human samples, making it an ideal choice for Western blot applications.CHD4, also known as chromodomain helicase DNA-binding protein 4, is involved in various cellular processes such as DNA repair, transcriptional regulation, and chromatin remodeling. Dysregulation of CHD4 has been linked to cancer progression, making it a promising target for cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CHD4 Antibody
SKU:CAB11574
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ELAE VEEN KKMS QPGS PSPK TPTP STPG DTQP NTPA PVPP AEDG IKIE ENSL KEEE SIEG EKEV KSTA PETA IECT QAPA PASE DEKV VVEP PEGE EKVE KAEV KERT EEPM ETEP KGAA DVEK VEEK SAID LTPI VVED KEEK KEEE EKKE VMLQ NGET PKDL NDEK QKK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:100 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CHD-4, Mi-2b, SIHIWES, Mi2-BETA, CHD4
Positive Sample:HeLa, Jurkat, 293T, HT-1080
Cellular Localization:Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:218kDa
Observed MW:280kDa

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID1108
RRIDAB_2758604
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CHD4 Rabbit pAb (CAB11574) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver using CHD4 Rabbit pAb (CAB11574) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver using CHD4 Rabbit pAb (CAB11574) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using CHD4 Rabbit pAb (CAB11574) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U20S cells using CHD4 Rabbit pAb (CAB11574) at dilution of 1:100. Blue: DAPI for nuclear staining.
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