The CENPC Monoclonal Antibody (CAB3975) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is produced in rabbits and is highly reactive with human samples, making it a reliable tool for researchers studying centromere function in various cell types.CENPC is a key protein involved in centromere assembly and function, playing a critical role in chromosome segregation during cell division. Mutations or dysregulation of CENPC have been linked to chromosomal instability and cancer development, making it an important target for cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CENPC Monoclonal Antibody
SKU:
CAB3975
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC2097
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MIF2, hcp-4, CENP-C, CENPC1, CENPC
Positive Sample:
HeLa, Jurkat
Cellular Localization:
Cytosol, Nuclear Body, Nucleoplasm.
Calculated MW:
107kDa
Observed MW:
107kDa
Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.
Purification Method
Affinity purification
Gene ID
1060
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CENPC Rabbit mAb (CAB3975) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb (CAB3975) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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