KO Validated Antibodies 1

[KO Validated] HP1 alpha/CBX5 Rabbit Polyclonal Antibody (CAB12577)

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SKU:
CAB12577
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse
Synonyms:
HP1, HP1A, HEL25, X5
Research Use:
Immunology
Frequently bought together:

Description

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[KO Validated] HP1 alpha/CBX5 Antibody (CAB12577)

The [KO Validated] HP1 alpha/CBX5 Antibody (CAB12577) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets CBX5 in human samples and has been validated for use in Western blot applications.CBX5, also known as HP1 alpha, is a member of the heterochromatin protein 1 (HP1) family and plays a crucial role in gene silencing and heterochromatin formation. It is involved in various cellular processes, including cell differentiation, DNA repair, and transcriptional regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] HP1 alpha/CBX5 Antibody
SKU:CAB12577
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGKK TKRT ADSS SSED EEEY VVEK VLDR RVVK GQVE YLLK WKGF SEEH NTWE PEKN LDCP ELIS EFMK KYKK MKEG ENNK PREK SESN KRKS NFSN SADD
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HP1, HP1A, HEL25, X5
Positive Sample:HeLa, Jurkat, SH-SY5Y, A-549, MCF7, Mouse brain, 293T
Cellular Localization:Chromosome, Nucleus, Centromere.
Calculated MW:22kDa
Observed MW:22kDa

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Purification MethodAffinity purification
Gene ID23468
RRIDAB_2759419
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] HP1 alpha/CBX5 Rabbit pAb (CAB12577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from wild type (WT) and HP1 alpha/HP1 alpha/CBX5 knockout (KO) 293T cells, using [KO Validated] HP1 alpha/CBX5 Rabbit pAb (CAB12577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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