Name: Anti-CBFA2T3 Antibody (CAB14712)
Brand: Developmental Biology
SKU: CAB14712
Availability: OutOfStock

Antibody Name:	Anti-CBFA2T3 Antibody
Antibody SKU:	CAB14712
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 305-390 of human CBFA2T3 (NP_005178.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	K-562, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 305-390 of human CBFA2T3 (NP_005178.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DRDP LHPE HLSK RPCT LNPA QRYS PSNG PPQP TPPP HYRL EDIA MAHH FRDA YRHP DPRE LRER HRPL VVPG SRQE EVID HKLT ER
Gene ID:	863
Uniprot:	O75081
Cellular Location:	Golgi apparatus membrane, Nucleus, nucleolus, nucleoplasm
Calculated MW:	7kDa/62kDa/63kDa/71kDa
Observed MW:	60kDa

Synonyms:

CBFA2T3, ETO2, MTG16, MTGR2, RUNX1T3, ZMYND4

Background:

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants.

UniProt Protein Function:	Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway (PubMed:25974097). Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (PubMed:23840896, PubMed:25974097). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation (PubMed:15231665).
NCBI Summary:	This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
UniProt Code:	O75081
NCBI GenInfo Identifier:	28872803
NCBI Gene ID:	863
NCBI Accession:	NP_005178.4
UniProt Secondary Accession:	O75081,O60615, O60616, O60617, O75082, O75107, O75108 Q0P5Z6, Q6P5W6, D3DX78,
UniProt Related Accession:	O75081
Molecular Weight:	7,436 Da
NCBI Full Name:	protein CBFA2T3 isoform 1
NCBI Synonym Full Names:	CBFA2/RUNX1 translocation partner 3
NCBI Official Symbol:	CBFA2T3
NCBI Official Synonym Symbols:	ETO2; MTG16; MTGR2; ZMYND4; RUNX1T3
NCBI Protein Information:	protein CBFA2T3
UniProt Protein Name:	Protein CBFA2T3
UniProt Synonym Protein Names:	MTG8-related protein 2; Myeloid translocation gene on chromosome 16 protein; hMTG16; Zinc finger MYND domain-containing protein 4
UniProt Gene Name:	CBFA2T3

Western blot analysis of extracts of various cell lines, using CBFA2T3 antibody (CAB14712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

CBFA2T3 Antibody (PACO05592)

CBFA2T3 Antibody (PACO08206)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)