CASTOR1 Polyclonal Antibody (CAB20710)

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SKU:
CAB20710
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
GATSL3, CASTOR1
Frequently bought together:

Description

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CASTOR1 Polyclonal Antibody (CAB20710)

The CASTOR1 Polyclonal Antibody (CAB20710) is a high-quality antibody developed for reliable detection and analysis of target proteins. Castor1 is a key regulator of autophagy, a cellular process crucial for maintaining cellular homeostasis and dealing with stress conditions. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.The Castor1 protein plays a critical role in the autophagy pathway, helping to regulate the process of self-degradation in response to various stimuli such as nutrient deprivation or infection.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CASTOR1 Polyclonal Antibody
SKU:CAB20710
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MELH ILEH RVRV LSVA RPGL WLYT HPLI KLLF LPRR SRCK FFSL TETP EDYT LMVD EEGF KELP PSEF LQVA EATW LVLN VSSH SGAA VQAA GVTK IARS VIAP LAEH HVSV LMLS TYQT DFIL VREQ DLSV VIHT LAQE FDIY REVG GEPV PVTR DDSS NGFP RTQH GPSP TVHP IQSP QNRF CVLT LDPE TLPA IATT LIDV LFYS HSTP KEAA SSSP EPSS ITFF AFSL IEGY ISIV MDAE TQKK FPSD LLLT SSSG ELWR MVRI GGQP LGFD ECGI VAQI AGPL AAAD ISAY YIST FNFD HALV PEDG IGSV IEVL QRRQ EGLA S
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GATSL3, CASTOR1
Positive Sample:Jurkat, Mouse lung
Cellular Localization:Cytosol.
Calculated MW:36kDa
Observed MW:39kDa

Enables arginine binding activity and identical protein binding activity. Involved in cellular response to L-arginine and negative regulation of TORC1 signaling. Located in cytosol. Colocalizes with GATOR2 complex.

Purification MethodAffinity purification
Gene ID652968
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using CASTOR1 Rabbit pAb (CAB20710) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using CASTOR1 Rabbit pAb (CAB20710) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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