Cell Death Antibodies 1

Caspase-8 Rabbit Polyclonal Antibody (CAB0215)

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SKU:
CAB0215
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8
Research Use:
Cell Death
Frequently bought together:

Description

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Caspase-8 Antibody (CAB0215)

The Caspase-8 Antibody (CAB0215) is a high-quality antibody developed for reliable detection and analysis of target proteins. Caspase-8 is a critical mediator of apoptosis, playing a key role in the initiation of the extrinsic apoptosis pathway. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications.By binding specifically to Caspase-8, this antibody allows for the detection and analysis of this important protein in a variety of cell types.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Caspase-8 Antibody
SKU:CAB0215
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:EEQP YLEM DLSS PQTR YIPD EADF LLGM ATVN NCVS YRNP AEGT WYIQ SLCQ SLRE RCPR GDDI LTIL TEVN YEVS NKDD KKNM GKQM PQPT FTLR KKLV FPSD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8
Positive Sample:C2C12, Jurkat,
Cellular Localization:Cytoplasm.
Calculated MW:55kDa
Observed MW:57kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Purification MethodAffinity purification
Gene ID841
RRIDAB_2757029
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Caspase-8 Rabbit pAb (CAB0215) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using Caspase-8 Rabbit pAb (CAB0215) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using Caspase-8 Rabbit pAb (CAB0215) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using Caspase-8 Rabbit pAb (CAB0215) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of HepG2 cells using Caspase-8 Rabbit pAb (CAB0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using Caspase-8 Rabbit pAb (CAB0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using Caspase-8 Rabbit pAb (CAB0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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