Cell Biology Antibodies 11

CAPN5 Rabbit Polyclonal Antibody (CAB7428)

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SKU:
CAB7428
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
VRNI, ADNIV, HTRA3, nCL-3, CAPN5
Research Use:
Cell Biology
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Description

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CAPN5 Antibody (CAB7428)

The CAPN5 Antibody (CAB7428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to calpain-5, this antibody enables accurate detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and cell signaling pathways.Calpain-5, also known as calcium-dependent cysteine protease, is a key player in regulating cell growth and survival through its ability to cleave specific protein targets. Its diverse functions make it a promising target for research into diseases such as cancer, neurodegenerative disorders, and diabetes.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CAPN5 Antibody
SKU:CAB7428
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GAWT LHED PRQN RGGG CINH KDTF FQNP QYIF EVKK PEDE VLIC IQQR PKRS TRRE GKGE NLAI GFDI YKVE ENRQ YRMH SLQH KAAS SIYI NSRS VFLR TDQP EGRY VIIP TTFE PGHT GEFL LRVF TDVP SNCR ELRL DEPP HTCW SSLC GYPQ LVTQ VHVL GAAG LKDS PTGA NSYV IIKC EGDK VRSA VQKG TSTP EYNV KGIF YRKK LSQP ITVQ VWNH RVLK DEFL GQVH LKAD PDNL QALH TLHL RDRN SRQP SNLP GTVA VHIL SSTS LMAV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:VRNI, ADNIV, HTRA3, nCL-3, CAPN5
Positive Sample:SKOV3, Mouse thymus, Mouse brain, Mouse spinal cord
Cellular Localization:Cell Surface, Cytoplasm, Extracellular Exosome, Focal Adhesion.
Calculated MW:73kDa
Observed MW:73kDa

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

Purification MethodAffinity purification
Gene ID726
RRIDAB_2767956
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CAPN5 Rabbit pAb (CAB7428) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using CAPN5 Rabbit pAb (CAB7428) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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