Cell Biology Antibodies 17
Calponin Rabbit Monoclonal Antibody (CAB3734)
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- SKU:
- CAB3734
- Product Type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human, Mouse, Rat
- Synonyms:
- SMCC, Sm-Calp, HEL-S-14, Calponin
- Research Use:
- Cell Biology
Frequently bought together:
Description
Calponin Monoclonal Antibody (CAB3734)
The Calponin Monoclonal Antibody (CAB3734) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in various research applications, such as immunohistochemistry and immunofluorescence.Calponin is a key regulator of actin cytoskeleton dynamics and plays a crucial role in cell migration, adhesion, and contraction. Dysregulation of calponin expression has been linked to a variety of diseases, including cardiovascular disorders and cancer.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | Calponin Monoclonal Antibody |
| SKU: | CAB3734 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Clone Number: | ARC0232 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||||
| Sequence: | PLDQ ATIS LQMG TNKG ASQA GMTA PGTK RQIF EPGL GMEH CDTL NVSL QMGS NKGA SQRG MTVY GLPR QVYD PKYC LTPE YPEL GEPA HNHH AHNY YNSA | ||||||||
| Tested Applications: | WB IHC-P ELISA IF-P | ||||||||
| Recommended Dilution: |
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| Synonyms: | SMCC, Sm-Calp, HEL-S-14, Calponin |
| Positive Sample: | Mouse lung, Mouse kidney, Mouse heart, Rat lung, Rat uterus |
| Cellular Localization: | Cytoskeleton, Focal Adhesion. |
| Calculated MW: | 33kDa |
| Observed MW: | 35kDa |
Predicted to enable actin binding activity. Involved in negative regulation of vascular associated smooth muscle cell proliferation. Located in cytoskeleton.
| Purification Method | Affinity purification |
| Gene ID | 1264 |
| RRID | AB_2863131 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using Calponin Rabbit mAb (CAB3734) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Calponin Rabbit mAb (CAB3734) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Calponin Rabbit mAb (CAB3734) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Calponin Rabbit mAb (CAB3734) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Calponin Rabbit mAb (CAB3734) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using Calponin Rabbit mAb (CAB3734) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Confocal imaging of paraffin-embedded Rat small intestine tissue using Calponin Rabbit mAb (CAB3734, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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