Epigenetics & Nuclear Signaling Antibodies 3

BRD3 Rabbit Polyclonal Antibody (CAB2277)

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SKU:
CAB2277
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Rat
Synonyms:
ORFX, FSHRG2, RING3L, BRD3
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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BRD3 Antibody (CAB2277)

The BRD3 Antibody (CAB2277) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for detecting and analyzing BRD3 protein in human samples. Validated for use in Western blot applications, this antibody offers high reactivity and specificity for studying BRD3 in various cell types.BRD3 is a member of the bromodomain and extraterminal domain (BET) family of proteins, known for their role in gene regulation and transcriptional activation. BRD3 specifically plays a crucial role in cell proliferation, differentiation, and oncogenesis, making it a promising target for cancer research and therapeutic development.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:BRD3 Antibody
SKU:CAB2277
Size:20μL, 100μL
Reactivity:Human, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PAPK GKGR KPAA GAQS AGTQ QVAA VSSV SPAT PFQS VPPT VSQT PVIA ATPV PTIT ANVT SVPV PPAA APPP PATP IVPV VPPT PPVV KKKG VKRK ADTT TPTT SAIT ASRS ESPP PLSD PKQA KVVA RRES GGRP IKPP KKDL EDGE VPQH AGKK
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ORFX, FSHRG2, RING3L, BRD3
Positive Sample:Jurkat, HeLa, HepG2, DU145, BT-474
Cellular Localization:Nucleus.
Calculated MW:80kDa
Observed MW:115kDa

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

Purification MethodAffinity purification
Gene ID8019
RRIDAB_2764269
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using BRD3 Rabbit pAb (CAB2277) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis using BRD3 Rabbit pAb (CAB2277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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