Cell Death Antibodies 1

Bid Rabbit Polyclonal Antibody (CAB0210)

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SKU:
CAB0210
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
FP497, Bid
Research Use:
Cell Death
Frequently bought together:

Description

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Bid Antibody (CAB0210)

The Bid Antibody (CAB0210) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in Western blot applications, demonstrating high reactivity with human samples. By binding to the BID protein, this antibody enables researchers to detect and analyze BID expression in a variety of cell types, making it an ideal tool for studies in cell biology, cancer research, and apoptosis regulation.BID, a member of the Bcl-2 protein family, functions as a critical mediator in the apoptotic pathway, linking extrinsic death receptor signaling to the intrinsic mitochondrial pathway.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Bid Antibody
SKU:CAB0210
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDCE VNNG SSLR DECI TNLL VFGF LQSC SDNS FRRE LDAL GHEL PVLA PQWE GYDE LQTD GNRS SHSR LGRI EADS ESQE DIIR NIAR HLAQ VGDS MDRS IPPG LVNG LALQ LRNT SRSE EDRN RDLA TALE QLLQ AYPR DMEK EKTM LVLA LLLA KKVA SHTP SLLR DVFH TTVN FINQ NLRT YVRS LARN GMD
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FP497, Bid
Positive Sample:Jurkat, Rat thymus, Mouse liver, Mouse heart, 293T
Cellular Localization:Cytoplasm, Mitochondrion Membrane, Mitochondrion Membrane.
Calculated MW:22kDa
Observed MW:20kDa//15kDa

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.

Purification MethodAffinity purification
Gene ID637
RRIDAB_2757024
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Bid Rabbit pAb (CAB0210) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from Rat thymus, using Bid Rabbit pAb (CAB0210) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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