Cell Death Antibodies 1
Anti-Bid Antibody (CAB0210)
- SKU:
- CAB0210
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-Bid Antibody |
| Antibody SKU: | CAB0210 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human Bid (NP_001187.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human Bid (NP_001187.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDCE VNNG SSLR DECI TNLL VFGF LQSC SDNS FRRE LDAL GHEL PVLA PQWE GYDE LQTD GNRS SHSR LGRI EADS ESQE DIIR NIAR HLAQ VGDS MDRS IPPG LVNG LALQ LRNT SRSE EDRN RDLA TA |
| Gene ID: | 637 |
| Uniprot: | P55957 |
| Cellular Location: | Cytoplasm, Mitochondrion membrane, Mitochondrion membrane |
| Calculated MW: | 11kDa/14kDa/21kDa/26kDa |
| Observed MW: | 22kDa |
| Synonyms: | BID, FP497 |
| Background: | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| UniProt Protein Function: | BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found. |
| UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 22q11.1 Cellular Component: cytosol; membrane; mitochondrial outer membrane; mitochondrion Molecular Function:death receptor binding; protein binding Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction; induction of apoptosis via death domain receptors; neuron apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization; positive regulation of protein oligomerization; regulation of apoptosis; release of cytochrome c from mitochondria |
| NCBI Summary: | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55957 |
| NCBI GenInfo Identifier: | 2493285 |
| NCBI Gene ID: | 637 |
| NCBI Accession: | P55957.1 |
| UniProt Secondary Accession: | P55957,Q549M7, Q71T04, Q7Z4M9, Q8IY86, |
| UniProt Related Accession: | P55957 |
| Molecular Weight: | 11,263 Da |
| NCBI Full Name: | BH3-interacting domain death agonist |
| NCBI Synonym Full Names: | BH3 interacting domain death agonist |
| NCBI Official Symbol: | BID |
| NCBI Official Synonym Symbols: | FP497 |
| NCBI Protein Information: | BH3-interacting domain death agonist |
| UniProt Protein Name: | BH3-interacting domain death agonist |
| UniProt Synonym Protein Names: | p22 BID; BID |
| Protein Family: | BH3-interacting domain death agonist |
| UniProt Gene Name: | BID |
| UniProt Entry Name: | BID_HUMAN |
 | Western blot analysis of extracts of HL-60 cells, using Bid Antibody (CAB0210). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunofluorescence analysis of C6 cells using Bid antibody (CAB0210) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Bid antibody (CAB0210) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using Bid antibody (CAB0210) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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