Cell Biology Antibodies 10

BHLHE40 Rabbit Polyclonal Antibody (CAB6534)

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SKU:
CAB6534
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
DEC1, HLHB2, BHLHB2, Clast5, SHARP2, STRA13, Stra14, SHARP-2, BHLHE40
Research Use:
Cell Biology
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Description

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BHLHE40 Antibody (CAB6534)

The BHLHE40 Antibody (CAB6534) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blot applications. By binding to the BHLHE40 protein, this antibody allows for the detection and analysis of BHLHE40 in various cell types, making it ideal for studies in molecular biology and developmental biology.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:BHLHE40 Antibody
SKU:CAB6534
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LQSG LQAG ELSG RNVE TGQE MFCS GFQT CARE VLQY LAKH ENTR DLKS SQLV THLH RVVS ELLQ GGTS RKPS DPAP KVMD FKEK PSSP AKGS EGPG KNCV PVIQ RTFA HSSG EQSG SDTD TDSG YGGE SEKG DLRS EQPC FKSD HGRR FTMG ERIG AIKQ ESEE PPTK KNRM QLSD DEGH
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DEC1, HLHB2, BHLHB2, Clast5, SHARP2, STRA13, Stra14, SHARP-2, BHLHE40
Positive Sample:HepG2, Mouse kidney
Cellular Localization:Cytoplasm, Nucleus, Nucleolus.
Calculated MW:46kDa
Observed MW:48kDa

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation.

Purification MethodAffinity purification
Gene ID8553
RRIDAB_2767127
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using BHLHE40 Rabbit pAb (CAB6534) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung using BHLHE40 Rabbit pAb (CAB6534) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat colon using BHLHE40 Rabbit pAb (CAB6534) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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