Name: Anti-BHLHE40 Antibody (CAB6534)
Brand: Cell Biology Antibodies 10
SKU: CAB6534
Availability: OutOfStock

Antibody Name:	Anti-BHLHE40 Antibody
Antibody SKU:	CAB6534
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 173-412 of human BHLHE40 (NP_003661.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 173-412 of human BHLHE40 (NP_003661.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	THLH RVVS ELLQ GGTS RKPS DPAP KVMD FKEK PSSP AKGS EGPG KNCV PVIQ RTFA HSSG EQSG SDTD TDSG YGGE SEKG DLRS EQPC FKSD HGRR FTMG ERIG AIKQ ESEE PPTK KNRM QLSD DEGH FTSS DLIS SPFL GPHP HQPP FCLP FYLI PPSA TAYL PMLE KCWY PTSV PVLY PGLN ASAA ALSS FMNP DKIS APLL MPQR LPSP LPAH PSVD SSVL LQAL KPIP PLNL ETKD
Gene ID:	8553
Uniprot:	O14503
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	45kDa
Observed MW:	48kDa

Synonyms:	BHLHE40, BHLHB2, Clast5, DEC1, HLHB2, SHARP-2, SHARP2, STRA13, Stra14
Background:	This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation.

UniProt Protein Function:	BHLHB2: May function as a transcriptional factor to modulate chondrogenesis in response to the cAMP pathway.
UniProt Protein Details:	Protein type:Nucleolus; Transcription regulation Chromosomal Location of Human Ortholog: 3p26.1 Cellular Component: Golgi apparatus; nucleolus; nucleus Molecular Function:bHLH transcription factor binding; MRF binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription corepressor activity Biological Process: circadian regulation of gene expression; entrainment of circadian clock by photoperiod; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]
UniProt Code:	O14503
NCBI GenInfo Identifier:	20137254
NCBI Gene ID:	8553
NCBI Accession:	O14503.1
UniProt Secondary Accession:	O14503,Q96TD3,
UniProt Related Accession:	O14503
Molecular Weight:	45,510 Da
NCBI Full Name:	Class E basic helix-loop-helix protein 40
NCBI Synonym Full Names:	basic helix-loop-helix family member e40
NCBI Official Symbol:	BHLHE40
NCBI Official Synonym Symbols:	DEC1; HLHB2; BHLHB2; Clast5; SHARP2; STRA13; Stra14; SHARP-2
NCBI Protein Information:	class E basic helix-loop-helix protein 40
UniProt Protein Name:	Class E basic helix-loop-helix protein 40
UniProt Synonym Protein Names:	Class B basic helix-loop-helix protein 2; bHLHb2; Differentially expressed in chondrocytes protein 1; DEC1; Enhancer-of-split and hairy-related protein 2; SHARP-2; Stimulated by retinoic acid gene 13 protein
UniProt Gene Name:	BHLHE40

	Western blot analysis of extracts of various cell lines, using BHLHE40 antibody (CAB6534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using BHLHE40 antibody (CAB6534) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using BHLHE40 antibody (CAB6534) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using BHLHE40 antibody (CAB6534). Blue: DAPI for nuclear staining.