Signal Transduction Antibodies 3

BAP1 Rabbit Polyclonal Antibody (CAB6533)

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SKU:
CAB6533
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
UBM2, UVM2, KURIS, TPDS1, UCHL2, hucep-6, HUCEP-13, BAP1
Research Use:
Signal Transduction
Frequently bought together:

Description

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BAP1 Antibody (CAB6533)

The BAP1 Antibody (CAB6533) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for BAP1 in human samples and is validated for use in Western blot applications.BAP1, also known as BRCA1-associated protein 1, is a tumor suppressor gene that plays a key role in regulating cell proliferation and preventing the formation of tumors. Mutations in the BAP1 gene have been linked to various cancers, including mesothelioma and uveal melanoma.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:BAP1 Antibody
SKU:CAB6533
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MNKG WLEL ESDP GLFT LLVE DFGV KGVQ VEEI YDLQ SKCQ GPVY GFIF LFKW IEER RSRR KVST LVDD TSVI DDDI VNNM FFAH QLIP NSCA THAL LSVL LNCS SVDL GPTL SRMK DFTK GFSP ESKG YAIG NAPE LAKA HNSH ARPE PRHL PEKQ NGLS AVRT MEAF HFVS YVPI TGRL FELD GLKV YPID HGPW GEDE EWTD KARR VIME RIGL ATAG EPYH DIRF NLMA VVPD RRIK YEAR LHVL KVNR QTVL EALQ QLIR VTQP EL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:UBM2, UVM2, KURIS, TPDS1, UCHL2, hucep-6, HUCEP-13, BAP1
Positive Sample:Mouse testis, Mouse brain, Rat brain
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:80kDa
Observed MW:95kDa

This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma.

Purification MethodAffinity purification
Gene ID8314
RRIDAB_2767126
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using BAP1 Rabbit pAb (CAB6533) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using BAP1 Rabbit pAb (CAB6533) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis using BAP1 Rabbit pAb (CAB6533) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using BAP1 Rabbit pAb (CAB6533) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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